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KMID : 0368420070500030315
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Journal of Plant Biology
2007 Volume.50 No. 3 p.315 ~ p.320
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In vitro biosynthesis of strictosidine usinglonicera japonica leaf extracts and recombinant yeast
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Nam Kang-Hee
Chung Hwa-Jee
Jeon Eo-Jin
Park Mi-Kyung
Yim Yong-Hyeon
Liu Jang-Ryol
Park Jeong-Ho
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Abstract
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Strictosidine is a key intermediate in the biosynthesis of the terpenoid indole alkaloid (T1A) pathway. It results from a condensation reaction, catalyzed by strictosidine synthase (STR), between tryptamine and secologanin. We have now developed a useful method, based on enzyme-assisted synthesis, to produce strictosidine. Our procedure utilizes leaf extracts from Japanese honeysuckleLonicera japonica Thunb. as a secologanin source. In these experiments, an enzyme extract was prepared from transgenic yeastSaccharomyces cerevisiae that expresses theCatharanthus roseus STR (CrSTR) coding region. Strictosidine was then isolated with a 38% yield based on the initial amount of tryptamine in the enzymatic reaction.
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KEYWORD
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Catharanthus roseus, honeysuckle Lonicera japonica, secologanin, strictosidine, strictosidine synthase
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